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Poly(I:C) Lipofectin Calculation
Unit/Mass To Mass/Unit Calculation
Poly (I:C) Lipofectin Calculation
Multiwell plates
Growth Area
(cm
2
)
Number of Cells
Lipofectin™ Reagent (µl/well)
Volume
Serum to Add
Total Assay Volume
96-well
0.3
3 x 10
4
1 µl
99 µl
100 µl
24-well
2
5 x 10
4
2 µl
98 µl
100 µl
12-well
4
6 x 10
4
2 µl
98 µl
100 µl
6-well
9.5
7 x 10
4
4 µl
96 µl
100 µl
Enter concentration in milligram/ml:
µg/ml =
ng/ml=
pg/ml=
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