Rhesus/Cyno Interferon Alpha 2

Product Information: The cDNA for Rhesus/Cyno IFN-Alpha 2 was cloned from the Rhesus cell line LLC-MK2. This was found to be identical to the putative Rhesus IFN-Alpha 2 (XM_001107516) identified as part of the Rhesus genome project (http://www.hgsc.bcm.tmc.edu/projects/rmacaque). There are 14 AA differences in the mature protein (2 in the signal peptide) between the Rhesus and Human Alpha A (2a) sequence. There are 13 AA differences in the mature protein (2 in the signal peptide) between the Rhesus and Human Alpha 2 (2b) sequence. This is in accord with a previous report [Villinger et al. (1995) J. Immunol. 155:3946] stating that there are 15-16 amino acids different between the Rhesus IFN alpha 2 and human alpha 2 (2b) and human alpha A(2a) respectively

This Rhesus (Macaca mulatta) sequence is also identical to a Cynomologous (Macaca fascicularis) IFN-Alpha 2 cloned from cDNA isolated from primary hepatocytes (PBL unpublished observation).

Comparison of the Mature Sequences of Human and Rhesus IFN-Alpha 2

Rhesus   1 CDLPQTHSLG NRRTLMLLAQ MRRISLFFCL KDRHDFEFPQ EEFGNQFQKA QTIPVLHEMI

Human    1 .......... S........ ........S. .......G... .......... E.........

Rhesus  61 QQTFNLFSTK DSSAAWDETL LNKFYTELYQ QLNDLEACVM QEMGVTETPL MNKNSILAVR

Human   61 ..I....... .......... .D........ .........I .GV....... .KED......

Rhesus 121 KYFQRITLYL KEKKYSLCAW EVVRAEIMRS FSLSTNLQES LRSKE

 Human 121 .......... ......P... .......... .......... .....

Figure 2: Activity of Rhesus/Cyno and Human IFN Alpha A on human cells (A549) challenged with EMCV.

This Product is for RESEARCH USE ONLY and is not for sale or use in any commercial kit or for use in the preparation of any commercial antibody. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE, OR USE IN HUMANS.