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VeriKineTM Cynomolgus/Rhesus IFN-Alpha Serum ELISA Kit
Product Number: 46100-1
Size: Single-plate kit

Product Information

Kit Components
Contents
Plate (s)
1
Plate Sealers
4
A : Wash Solution Concentrate
50 mL
B: Rhesus/Cynomolgus IFN Alpha 2 Standard 10,000pg/ml
0.5 mL
C: Sample Buffer
30 mL
D: Antibody Concentrate
1 vial
E: HRP Conjugate Concentrate
1 vial
F: Assay Diluent
30 mL
G: TMB Substrate Solution
15 mL
H: Stop Solution
15 mL

Lot Specific Preparations Refer to Cynomolgus/Rhesus IFN Alpha Serum ELISA Protocol for full instructions.

Antibody Concentrate Preparation

Micro-plate Strips Used
2
4
6
8
10
12
D:Antibody Concentrate (µl)
High Sensitivity 25-1600 pg/ml
            
F: Assay Diluent (ml)
2.0
4.0
6.0
8.0
10.0
12.0

HRP Conjugate Concentrate Preparation

Micro-plate Strips Used
2
4
6
8
10
12
E:HRP Conjugate Concentrate (µl)
High Sensitivity 25-1600 pg/ml
            
F: Assay Diluent(ml)
2.0
4.0
6.0
8.0
10.0
12.0

 

Performance Characteristics
Intra-Assay CV: ≤ 8 % (sample diluent, Cynomolgus serum, and Tissue culture media + 10% FBS)
Inter-Assay CV: ≤10% (sample diluent), ≤ 15% (Cynomolgus serum), and ≤ 10% Tissue culture media + 10% FBS
Spike Recovery:  >90% .

This Product Is For Research Use Only. Not For Use in Diagnostic Procedures.

Rev. 00