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VeriKine?Human IFN-Alpha ELISA Kit
Product Number: 41100-2
Size: Five-plate kit

Product Information

Kit Components
Contents
Plate (s)
5
Plate Sealers
20
A : Wash Solution Concentrate
250 mL
B: Human IFN Alpha Standard 10,000pg/ml
2.5 mL
C: Dilution Buffer
250 mL
D: Antibody Concentrate
1 vial
E: HRP Conjugate Concentrate
1 vial
F: HRP Conjugate Diluent
60 mL
G: TMB Substrate Solution
60 mL
H: Stop Solution
60 mL

Lot Specific Preparations Refer to Human IFN Alpha ELISA Protocol v.1.5 for full instructions.

Antibody Concentrate Preparation

Micro-plate Strips Used
2
4
6
8
10
12
D: Antibody Concentrate (µl)
High Sensitivity 12.5-500 pg/ml
            
 
Extended Range 156-5000 pg/ml
            
C: Dilution Buffer (ml)
2.0
4.0
6.0
8.0
10.0
12.0


HRP Conjugate Concentrate Preparation

Micro-plate Strips Used
2
4
6
8
10
12
E: HRP Conjugate Concentrate (µl)
High Sensitivity 12.5-500 pg/ml
            
 
Extended Range 156-5000 pg/ml
            
F: HRP Conjugate Diluent (ml)
2.0
4.0
6.0
8.0
10.0
12.0

Performance Characteristics
Intra-Assay CV: ≤ 8 % 
Inter-Assay CV: ≤ 8%
Spike Recovery:  >90%

This Product Is For Research Use Only. Not For Use in Diagnostic Procedures.

Rev. 01