Product Number: 31500-1
Size: 2 x 104 units
Product Information
Description: Rabbit Anti-Human Interferon Gamma
Contents: 2 x 104 neutralization units/vial
Volume: 0.1 ml
Bioactivity: 2 x 105 neutralization units/ml
Concentration: 0.174 mg/ml (estimate)
Form: neat serum diluted in phosphate buffered saline (PBS)
Antigen: recombinant human interferon gamma
Assay Used to Measure Bioactivity: One neutralization unit is the amount of antiserum required to neutralize one unit of human interferon gamma (Hu-IFN-γ) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol. 37, 755‑758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387‑394]. In this antiviral assay for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Hu-IFN-γ provided by the National Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14‑23]. This material is prepared specifically for effective neutralization of Hu-IFN-γ.
Suggested Applications: Western blot; immunoprecipitation; immunohistochemistry; ELISA
Please note that these applications are presented for suggested use only and have not been fully evaluated by PBL
Shipping Information
Shipping Conditions: Dry ice
Physical State of Product During Shipping: Frozen
Special Conditions/Comments: After receipt, this product should be kept at -20°C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.
This Product is for RESEARCH USE ONLY and is not for sale or use in any commercial kit.
NOT FOR DIAGNOSTIC OR THERAPEUTIC USE, OR USE IN HUMANS.
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