Product Information

Description:  Mouse Anti-Human Interferon Alpha/Beta R1

Clone: MMHB-1

Contents: 500 µg/vial

Concentration 0.5 mg/ml; after reconstitution with 1 ml sterile PBS

Form: Lyophilized from a 0.2 µm filtered solution in phosphate-buffered saline (PBS) containing 5% trehalose

Antigen:SF21 -derived Hu-IFN-α/β R1 extracellular domain

Isotype: Mouse IgG₁

Purification Method:Protein G affinity chromatography

Specificity:Detects human interferon alpha/beta R1; in direct ELISAs, this antibody shows no cross reactivity with Hu-IFN-γ R1, Hu-IFN-γ R2, Mu-IFN-α/β R2, Hu-IL-10 Rβ, Hu-IL-10 Rα or Hu-IL-20 Rβ

Tested Applications: Optimal dilutions should be determined by each laboratory for each application

Western Blot: 1 - 2 µg/ml with the appropriate secondary reagents to detect human IFN-α/β R1.

Flow Cytometry: Dilute antibody to 25 µg/ml and add 10 µl of this solution to 1 – 2.5 x 10⁵ cells in a total reaction volume not exceeding 200ml. The binding of the unlabeled antibody may be visualized by adding 10 µl of a 25 µg/ml stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to fluorochrome.

Direct ELISA: 0.5 - 1.0 µg/ml with the appropriate secondary reagents to detect human IFN-α/β R1.

Shipping Conditions: Wet Ice

Physical State of Product During Shipping: Lyophilized

Special Conditions: After receipt, this product should be kept at -20°C for retention of full activity. Upon reconstitution with sterile PBS, the antibody can be stored at 2-°C for 1 month without detectable loss of activity. Reconstituted antibody can also be aliquoted and frozen at -20° to -70°C in a manual defrost freezer for 6 months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

This Product is for RESEARCH USE ONLY and is not for sale or use in any commercial kit.
NOT FOR DIAGNOSTIC OR THERAPEUTIC USE, OR USE IN HUMANS.

v. 1.0