Product Number: 16100-1
Size: ≥1 x 105 units
Product Information
Description: Recombinant Cynomolgus Interferon alpha 2 (Cyno IFN-α2 [Ile 16])
Contents: ≥1 x 105 units / vial (MDBK/VSV)
Volume: 0.1 ml
Concentration: ≥ 1 x 106 units/ml (MDBK/VSV)
Buffer: Phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)
Endotoxin: < 1 EU/µg
Molecular Weight: 19.5 kDa
Purity: > 98%
Source: Cynomolgus (Macaca fascicularis) cDNA expressed in E. coli
Accession Number: None
Assays Used to Measure Bioactivity: Interferon was titrated in the Cytopathic Effect Inhibition (CPE) assays as listed. The protein has similar activity to Human IFN alpha 2 on human, rhesus and bovine cells.
Bovine (MDBK/VSV)- performed as described [Rubinstein et al. (1981) J. Virol. 37(2):755] The EC50 for interferon in this assay is ~5 U/ml. Specific Activity: (refer to lot specific C.O.A) x 108 units/mg.
Human (A549/EMCV)- performed as described [Budd et al. (1985) Canc. Chem. Pharm. 12:39]. The EC50 for interferon in this assay is ~1 U/ml. Specific Activity: (refer to lot specific C.O.A)x 108 units/mg.
Rhesus (LLC-MK2/VSV)- developed and performed at PBL Biomedical Laboratories. The EC50 for interferon in this assay is ~200 U/ml. Specific Activity: (refer to lot specific C.O.A) x 108 units/mg
As there is no international Monkey Interferon Alpha standard, the units are determined by use of a Human IFN Alpha A (Hu IFN-aA [2a]) laboratory standard calibrated to the international reference standard for Human Interferon Alpha A (Hu IFN-aA [2a]) provided by the National Institutes of Health [Meager et al. (2001). J Immunol Methods. 257(1-2):17-33.].
Shipping Information
Shipping Conditions: Dry ice
Physical State of Product During Shipping: Frozen
Special Conditions/Comments: After receipt, this product should be kept at -70°C or below for retention of full activity. Thaw product vial by incubation in cold tap water until just thawed - the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein, such as 0.1% bovine serum albumin (BSA). For more information on protein handling visit the PBL website at www.interferonsource.com.
Product Information The cDNA for Cyno IFN-a2 was cloned from Cynomolgus (Macaca fascicularis) primary hepatocytes. Two sequences were identified, the first was found to be identical to the putative Rhesus IFN-a2 (XM_001107516) PBL Cat No. 14110 (Rhesus/Cyno IFN Alpha 2). The second, PBL Cat No. 16100-1 (Cynomolgus IFN Alpha 2 [Ile 16]), may represent an allelic form which differs from the Rhesus/Cyno Alpha 2 sequence by 1 amino acid. This version has Isoleucine at position 16 while the Rhesus/Cyno Alpha 2 has Methionine at position 16. Both forms were identified multiple times from the hepatocytes.
Figure 1: Activity of Cyno IFN Alpha 2 [Ile 16] and Human IFN Alpha A on rhesus cells (LLC-MK2).
Figure 2: Activity of Cyno IFN Alpha 2 [Ile 16] and Human IFN Alpha A on human cells (A549).
This Product is for RESEARCH USE ONLY and is not for sale or use in any commercial kit or for use in the preparation of any commercial antibody. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE, OR USE IN HUMANS.
v. 1.0
| 
Cynomolgus Interferon Alpha 2 [Ile 16]