Product Number: 12501-1
Size: 20 µg
Product Information
Description: Recombinant Mouse Interferon Gamma (Mouse IFN-γ), carrier-free
Contents: 20 µg/vial
Volume: 0.1 ml
Concentration: 0.2 mg/ml
Buffer: phosphate buffered saline (PBS)
Molecular Weight: 15,521
Purity: ≥95%
Source: cDNA obtained from mouse leukocytes expressed in E. coli
Accession Number: K00083
Specific Activity: 1.02 x 107 units/mg
Assay Used to Measure Bioactivity: Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol. 37, 755‑758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387‑394]. In this antiviral assay for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for mouse interferon gamma (mouse IFN-γ) provided by the National Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119 , 14‑23]. Unit of activity measured on mouse L929 cells with encephalomyocarditis virus (EMCV).
Shipping Information
Shipping Conditions: Dry ice
Physical State of Product During Shipping: Frozen
Special Conditions/Comments: After receipt, this product should be kept at -70°C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.
This Product is for RESEARCH USE ONLY and is not for sale or use in any commercial kit.
NOT FOR DIAGNOSTIC OR THERAPEUTIC USE, OR USE IN HUMANS.
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Mouse Interferon Gamma, Carrier-Free