Product Information

Description: Human Growth Hormone Releasing Factor [hGRF(1-44)-NH₂M/]

COOH-Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-
Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Ser-Arg-
Gln-Gln-Gly-Glu-Ser-Asn-Gln-Glu-Arg-Gly-Ala-Arg-Ala-Arg-Leu-NH₂

C₂₁₅H₃₅₈N₇₂O₆₆S9CF₃COOH

Molecular Weight (as TFA salt): 6065.9

Contents: 1mg/vial, lyophilized

Purity: ≥97% by analytical HPLC  Lichrosorb RP8 [0.1M NaClO₄ (pH 2.5) - CH₃CN]

Characterization: Fast atom bombardment mass spectrometry:

Found: [M+H]+ 5040.5

Calculated:[M+H]+ 5040.8

Amino Acid Analysis:

Asp,4.08(4);Thr,0.94(1);Ser,3.75(4);Glu,7.40(7);Ala,5.00(5);Val,1.00(1);Met,0.90(1);
Ile,1.98(2);Leu,4.79(5);Gly,3.14(3);Tyr,1.97(2);Phe,0.96(1);Lys,1.75(2); Arg,5.94(6)

Biological Activity:

In Vitro: Cultured rat anterior pituitary cells were dispersed and incubated with hGRF(1-44)-NH2 for 4 hours at 37°C at 3.1-200pM.  Aliquots were assayed for Growth Hormone by standard double antibody RIA.

In Vivo: hGRF(1-44)-NH2 was injected into mice IV (retro-orbital, 0-30μg/kg) and bled at time intervals of 0, 5, 10, 15, 30 and 60 minutes (post-injection).  Aliquots were assayed for Growth Hormone by standard double antibody RIA.

Reconstitution: Reconstitute in 0.5 - 1.0 ml distilled water.

Shipping Information

Shipping Conditions: Dry ice

Physical State of Product During Shipping: Frozen

Special Conditions/Comments: After receipt, this product should be kept at -70°C or below for retention of full activity.  Centrifuge before use to insure material is at bottom of vial.  When reconstituting, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.

This Product is for RESEARCH USE ONLY and is not for sale or use in any commercial kit or for use in the preparation of any commercial antibody.
NOT FOR DIAGNOSTIC OR THERAPEUTIC USE, OR USE IN HUMANS.

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