Product Number: 11500-1
Size: 50 µg
Product Information
Description: Recombinant Human Interferon Gamma (Hu-IFN-γ)
Contents: 50 µg/vial
Buffer: phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)
Molecular Weight: 16,775
Purity: ≥95%
Source: cDNA obtained from human T lymphocyte mRNA expressed in E. coli
Accession Number: V00543
Assay Used to Measure Bioactivity: Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol. 37, 755‑758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387‑394]. In this antiviral assay for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for human interferon gamma (Hu-IFN-γ) provided by the National Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14‑23]. Unit of activity measured on H226 cells with vesicular stomatitis virus (VSV).
Shipping Information
Shipping Conditions: Dry ice
Physical State of Product During Shipping: Frozen
Special Conditions/Comments: After receipt, this product should be kept at -70°C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Dilute only in buffers containing protein such as 0.1% bovine serum albumin (BSA).
This Product is for RESEARCH USE ONLY and is not for sale or use in any commercial kit.
NOT FOR DIAGNOSTIC OR THERAPEUTIC USE, OR USE IN HUMANS.
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Human Interferon Gamma